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Alternative Tests
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Skin Irritation |
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Phototoxicity |
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Eye Irritation |
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Human Tests
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Skin Primary Irritation |
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Skin Cumulative Irritation |
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Sensitization |
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Stinging tests |
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Challenge Tests |
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Anti-microbial Efficacy |
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In vitro tests
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Suppression of melanin production in B16 melanoma cells |
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Suppression of tyrosinase biosynthesis/Inhibition of Tyrosinase Activity |
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Suppression of melanin production in a reconstructed skin model |
In vivo tests
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Suppression of UVB-initiated skin pigmentation |
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In vitro tests
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Activation of energy production (keratinocytes and fibroblasts) |
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Tolerances against oxidative stress (UVB, ROS) (keratinocytes and fibroblasts) |
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Stimulation of Collagen (Type I) production (fibroblasts) |
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Stimulation of Hyaluronic acid production (keratinocytes and fibroblasts) |
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Suppression of MMP-1 production/Inhibition of MMP-1 activity (fibroblasts) |
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Inhibition of elastase activity (fibroblasts) |
In vivo tests
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Wrinkle repair by the replica method (wrinkle depth, wrinkle area) |
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In vitro tests
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Stimulation of NMF in keratinocytes |
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Stimulation of ceramides in keratinocytes |
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Stimulation of ceramide in a reconstructed skin model |
In vivo tests
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Moisture tests (skin surface water content, transepidermal water loss) |
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Corneocyte diagnosis (area, desquamation, residual ratio of nucleated cell) |
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Anti-bacterial effect against P. acnes. |
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Inhibition of lipase activity in P. acnes. |
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Hair growth test by image analysis (rate of hair growth, diameter of a hair, ratio of anagen hair and telogen hair) |
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Use test |
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